Efficient separation of large particles and giant cancer cells using an isosceles trapezoidal spiral microchannel

Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors. Their size, however, complicates their isolation from mainstream tumor cell populations. Standard techniques like fluorescence-activated cell sorting (FACS) rely on fluorescent labeling, introducing potential challenges in subsequent PGCC analyses. In response, we developed the Isosceles Trapezoidal Spiral Microchannel (ITSμC), a […]

Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNA synthetase

Malaria poses an enormous threat to human health. With ever increasing resistance to currently deployed drugs, breakthrough compounds with novel mechanisms of action are urgently needed. Here, we explore pyrimidine-based sulfonamides as a new low molecular weight inhibitor class with drug-like physical parameters and a synthetically accessible scaffold. We show that the exemplar, OSM-S-106, has […]

Plasmonic digital PCR for discriminative detection of SARS-CoV-2 variants

We developed a novel strategy for discriminative detection of SARS-CoV-2 variants based on the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. This method consists of the gold nanofilm-based dPCR chip fabrication for ultrafast heating and cooling cycles by the plasmonic photothermal effect, the LED quencher immobilization through the interfacing […]

Microfluidic-based modeling of human tissue barriers

 Tissue barrier-on-chip, a type of organ-on-a-chip, replicates the physiological microenvironments of human tissue barriers on a microfluidic platform. This chip garners attention as a complementary solution to animal testing in the drug development process by precisely mimicking the core functions of tissue barriers, such as regulating substance transport, on a small scale. In this paper, […]

Material-Efficient Multimaterial Projection Micro-stereolithography Using Droplet-Based Resin Supply

This paper presents a material-efficient multimaterial projection micro-stereolithography (PμSL), a digital light processing (DLP) additive manufacturing process for printing microstructures. We present a droplet-based resin supply system to address the issue of excessive material waste of the multimaterial PμSL. By depositing droplets of different liquid resins, 3D printing of a microstructure can still be performed […]

ATP1A1/BCL2L1 predicts the response of myelomonocytic and monocytic acute myeloid leukemia to cardiac glycosides

Myelomonocytic and monocytic acute myeloid leukemia (AML) subtypes are intrinsically resistant to venetoclax-based regimens. Identifying targetable vulnerabilities would limit resistance and relapse. We previously documented the synergism of venetoclax and cardiac glycoside (CG) combination in AML. Despite preclinical evidence, the repurposing of cardiac glycosides (CGs) in cancer therapy remained unsuccessful due to a lack of […]